iELM1.0 predicts the linear motif mediated interactions associated with a protein, and when possible provides structural information for the binding interface. The user can investigate their protein(s) or protein interaction network of interest for linear-motif mediated interactions and thus achieve an improved insight into the system or mechanism under study
What is a linear motif?
A linear motif is a compact binding interface usually 3-10 amino acids in length with 205 residues contributing to the affinity of the interaction. This results in a tendency for SLiM-mediated interactions to be low affinity and transient. These motifs have diverse functions ranging from trafficking (i.e. nuclear localization motifs), to modulation (i.e. phosphorylation) to scaffolding (PDZ motifs in complex formation). For more information, we refer you to the following reviews by Diella et al. and Davey et al
Linear motif mediated interactions are extensively used in many vital cellular functions suggestings their identification and characterisation are necessary to acheive a proper understanding of the proteomes inherent complexity. iELM provides the first tool to study these motif-domain interactions in both a case-by-case manner and within large-scale interaction networks.
If you found this tool useful please cite:
Weatheritt RJ, Luck K, Petsalaki E, Davey NE, Gibson TJ. The identification of short linear motif-mediated interfaces within the human interactome. 2012 Apr 1;28(7):976-82 here
Weatheritt RJ, Jehl P, Dinkel H, Gibson TJ. iELM - a web server to explore short linear motif-mediated interactions. Nucleic Acids Res. 2012 Jul;40(Web Server issue):W364-9. here